PBIO 8100:
Plant Molecular and Cellular Biology I
|
Fall 2008 |
|
MWF 11:15 am Rm 1503 |
|
Thurs 9:30-10:45 am Rm 2507 |
| Instructors: | Kelly
Dawe 542-1658/Office - Plant Sciences 4611 Xiaoyu Zhang, 542-4822 -Plant Sciences 4409 |
| Grading: | Exams
are 75% (25% each) of the course grade. Discussion groups are 25% of the course grade. |
Discussion groups: Primary literature will be assigned and discussed on designated days. ***Each student is expected to understand the methods, interpretation of data, experimental design and controls, and choice of experimental system. You will be asked to voice your views and summarize figures or sections of the papers.
Assigned Readings: The assigned research papers are linked below. Lecture material will be derived primarily from review articles. NOTE: lecture notes are here to give you a glimpse of what will be discussed. I wouldn't print them out just yet because I may change them, and because I'll hand them out during class anyway.
Syllabus
| Week 1: Intro to Mendel | |
| 8/20 |
Lecture: Intro to Mendel (lecture) |
| 8/21 |
Paper:Creighton and McClintock (1931) (lecture). A correlation of cytological and genetical crossing over in Zea mays. Proc. Nat. Acad. Sci. 17, 492-497 |
| 8/22 | Lecture: Chromosomal mutations and Mapping (lecture) |
| Week 2: Point mutations, TDNA | |
| 8/25 |
Lecture: Point mutants (lecture notes) |
| 8/27 |
Paper: Kaya et al (2000). hosoba toge toge, a syndrome caused by a large chromosomal deletion associated with a T-DNA insertion in Arabidopsis. Plant Cell Physiol. 41, 1055-1066 |
| 8/28 |
Lecture: TDNA (lecture notes) |
| 8/29 |
Paper: Ishida et al (1996). High efficiency transformation of maize (Zea mays L.) mediated by Agrobacterium tumefaciens. Nature Biotechnology 14, 745 - 750 |
| Week 3: Tagging | |
| 9/1 | Holiday |
| 9/3 |
Lecture: TDNA tagging/reverse genetics/TILLing (Zhang lecture) |
| 9/4 | Paper: Alonso et al (2008). Genome-wide insertional mutagenesis of Arabidopsis thaliana. |
| 9/5 | Paper: |
| Week 4: Maternal and Dominant mutations | |
| 9/8 | Lecture: Dominant mutations (lecture) |
| 9/10 | Paper: Gosti et al (1999). ABI1 protein phosphatase 2C is a negative regulator of abscisic acid signaling. Plant Cell 11, 1897-1909 |
| 9/11 |
Lecture: chloroplast genetics (lecture) |
| 9/12 |
Paper: Shikanai et al (2001). The chloroplast clpP gene, encoding a proteolytic subunit of ATP-dependent protease, is indispensable for chloroplast development in tobacco. Plant Cell Physiol. 42, 264-73. |
| Week 5: Epistasis | |
| 9/15 | Lecture: Epistasis (lecture) |
| 9/17 |
Paper: Ng and Yanofsky (2001). Activation of the Arabidopsis B class homeotic genes by Apetala. Plant Cell 13, 739-753. |
| 9/18 |
Lecture: Suppressors (lecture) |
| 9/19 |
Paper: Luo and Oppenheimer (1999) Genetic control of trichome branch number in Arabidopsis: the roles of the FURCA loci. Development 126: 5547-5557 |
| Week 6: Reverse Genetics | |
| 9/22 |
review :EXAMPLE QUESTIONS |
| 9/24 |
Exam I |
| 9/26 | |
| 9/27 | Lecture: Activation tagging (lecture) |
| Week 7: Plant Genes | |
| 9/29 |
Paper: Busov et al (2003). Activation Tagging of a Dominant Gibberellin Catabolism Gene (GA 2-oxidase) from Poplar That Regulates Tree Stature. Plant Physiol 132, 1283-1292 |
| 10/1 |
Lecture: Genome duplication and the origin of redundancy (lecture) |
| 10/2 |
Lecture: Plant genes |
| 10/3 |
Paper: Hong et al (2003). Regulatory Elements of the Floral Homeotic Gene AGAMOUS Identified by Phylogenetic Footprinting and Shadowing. Plant Cell 15, 1296-1309 |
| Week 8: Genomes | |
| 10/6 | Lecture: Model genomes |
| 10/8 | Paper: |
| 10/9 |
Lecture: Comparative genomics |
| 10/10 | Paper: |
| Week 9: Micoarrays, sequencing | |
| 10/13 | Lecture: Microarrays |
| 10/15 | Paper: |
| 10/16 | Lecture: High throughput sequencing |
| 10/17 | Paper: |
| Week 10: TEs | |
| 10/20 | Lecture: DNA and RNA TEs |
| 10/22 | Paper: |
| 10/23 | Lecture: TEs as tools |
| 10/24 | Paper: |
| Week 11: Exam, Fall break | |
| 10/27 | Review |
| 10/29 | Exam |
| 10/30 | Fall break/Plant Center |
| 10/31 | Fall Break/Plant Center |
| Week 12: Centromeres and Telomeres | |
| 11/3 | Lecture: Centromeres |
| 11/5 | Paper: |
| 11/6 | Lecture: Telomeres |
| 11/7 | Paper: |
| Week 13: Epigenetics | |
| 11/10 | Lecture: Epigenetics |
| 11/12 | Paper: |
| 11/13 | Lecture: Epigenetics |
| 11/14 | Paper: |
| Week 14: Epigenetics | |
| 11/17 | Lecture: Epigenetics |
| 11/19 | Paper: |
| 11/20 | Lecture: Epigenetics |
| 11/21 | Paper: |
| Week 15: Thanksgiving | |
| 11/24 | Holiday |
| 11/26 | Holiday |
| 11/27 | Holiday |
| 11/28 | Holiday |
| Week 16: Flowering time, imprinting | |
| 12/1 | Lecture: Flowering |
| 12/3 | Paper: |
| 12/4 | Lecture: Imprinting |
| 12/5 | Paper: |
| Week 17: end | |
| 12/8 | Review |