4500/6500 COURSE SYLLABUS FALL 2008

Tuesdays and Thursdays, 12:30 PM to 1:45 PM

Room 1501 Plant Sciences Building

 

Aug     Tue      19

Introduction to recombinant DNA technology

 

Aug     Thr       21

DNA – function, properties, & extraction

 

Aug     Tue      26

PCR fundamentals

 

Aug     Thr       28

More on PCR

 

Sep     Tue      2

Basic molecular cloning – restriction enzymes

 

Sep     Thr       4

Basic molecular cloning – plasmids

 

Sep     Tue      9

Constructing complex plasmids

 

Sep     Thr       11

Recombination-based cloning methods

 

Sep     Tue      16

DNA sequencing – Sanger protocol

-(After class- Graduate students need to submit Webpaper topics)

-Receive Exam 1

Sep     Thr       18

New approaches to DNA sequencing

 

Sep     Tue      23

DNA microarrays & transcriptional profiling

Take home Exam 1 due

Sep     Thr       25

cDNA libraries & RT-PCR

-(After class- Select/assign Research Project Teams

Sep     Tue      30

More on DNA microarrays & in vitro gene synthesis

 

Oct      Thr       2

TBA (student’s choice)

 

Oct      Tue      7

TBA (student’s choice)

 

 

Oct      Thr       9

Introduction to Microbial Biotechnology

Introduction to BioEnergy project

Oct      Tue      14

Medical products and Recombinant Protein Usage

 

Oct      Thr       16

Expressing Protein in Bacteria

 

Oct      Tue      21       

Gene regulation and Yeast Expression

 

Oct      Thr       23

2-Hybrid system

(After class, graduate students stay for web page tutorial)

Receive Exam 2

Oct      Tue      28

Plant Expression System part 1-2

 

Oct      Thr       30

Viral expression/gene silencing     

Take home Exam 2 due.           

Nov     Tue      4

Plant Genetic Engineering /Genetic Modified Organism (GMO) part 1-2

-(After class, graduate students need to provide their web page CD, for approval)

Nov     Thr       6

Food Safety/GMO/ Biotechnology regulation

Class Discussion

Nov     Tue      11

Stem Cell Research & Drug

 

Nov     Thr       13

Grad. Student Web Page presentations

 

Nov     Tue      18

Animal Cloning

-(After class, all teams need to provide their 1st research proposal DRAFT on CD and hardcopy)

Nov     Thr       20

Functional Genomics part 1-2

 

Nov       25, 27          

 

NO CLASS

Dec     Tue      2

What’s new in Genomics and EpiGenomics

 

Dec     Thr      4

Proteomics

 

Dec     Tue      9

Metabolomics

Receive Take home Final Exam

 

Thu, Dec. 13, 12 to 3pm:   Final Exam due

                                             Final research proposal on CD and hardcopy due