Supervisor Zheng-Hua Ye
Lab Coordinator – Beth Richardson
Link to the PALM System
ABOUT THE LAB
New users of the facility are asked to meet with Dr Ye and Beth Richardson to discuss their research needs*. Emphasis in the lab is on ultrastructural studies of plants and fungi.
Cryo-techniques and immunocytochemistry are used
on a regular basis to study the host-pathogen interactions in
fungal diseases of plants, plant cell wall characterization, fungal
growth and development, and meiosis in maize.
The Plant Biology EM Lab maintains a Zeiss EM 902A Transmission
Electron Microscope (TEM) available for faculty and student research.
The lab is also equipped with 3 ultramicrotomes,
a cryostat, and other support equipment and supplies.
Since 1994, the EM lab has specialized in sample preparation
using the Balzers High Pressure Freezing Machine HPM 010. High pressure
freezing followed by freeze substitution (fs) has become the gold
standard for cryo-preservation of specimens. Plunge freezing/fs
and standard chemical fixation methods are also available for sample
Mims, CW, and Richardson, EA. 2008. Light and electron microscopy of the spermogonial stage of Gymnoconia peckiana, one of the causes of orange rust of Rubus. Canadian J Botany 86: 1-6.
Mims, CW. et al. 2007. Canadian J Botany 85: 926-934.
Lee, C. et al. 2007. The PARVUS gene is expressed in cells undergoing secondary wall thickening and essential for glucuronoxylan biosynthesis. Plant Cell Physiol. 48: 1659-1672.
Zhong, R. et al. 2007. The MYP transcription factor MYB46 is a direct target of SND1 and regulates secondary wall biosynthesis in Arabidopsis. Plant Cell 19: 2776-2792.
Zhong, R. et al. 2007. Planta 225: 1603-1611.
Pena, MJ. et al. 2007. Plant Cell 19: 549-563.
At the 2008 Microscopy & Microanalysis Meeting in Albuquerque, NM:
>> Diatome Poster Award. Biological Sciences - Second Place
WANT TO USE THE FACILITY OR CONTACT US?
For more information please contact Beth Richardson.
*Plant Biology faculty, students and technicians
should consult the department handbook for more information on
the laboratory and the equipment available for use.